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Local (gut) and systemic metabolism of rats is altered by consumption of raw bean (Phaseolus vulgaris L. var. athropurpurea)

Published online by Cambridge University Press:  09 March 2007

Carmen Cavallé de Moya ,
George Grant ,
Gema Frühbeck ,
Elena Urdaneta ,
María García ,
Florencio Marzo  and
Santiago Santidrián
Carmen Cavallé de Moya
Affiliation:
Department of Human Physiology, School of Medicine, University of Navarra, 31008 Pamplona, Spain
George Grant
Affiliation:
The Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB2 9SB, Scotland, UK
Gema Frühbeck
Affiliation:
Department of Human Physiology, School of Medicine, University of Navarra, 31008 Pamplona, Spain
Elena Urdaneta
Affiliation:
Department of Animal Physiology and Nutrition, Public University of Navarra, 31006 Pamplona, Spain
María García
Affiliation:
Department of Human Physiology, School of Medicine, University of Navarra, 31008 Pamplona, Spain
Florencio Marzo
Affiliation:
Department of Animal Physiology and Nutrition, Public University of Navarra, 31006 Pamplona, Spain
Santiago Santidrián*
Affiliation:
Department of Human Physiology, School of Medicine, University of Navarra, 31008 Pamplona, Spain
*
*Corresponding author: Dr Santiago Santidrián, fax +34 48 425 649, email santidrian@unav.es
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Abstract

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The composition of the raw legume Phaseolus vulgaris L. var. athropurpurea (PhVa) and its effects on the metabolism of young growing rats have been evaluated. The levels of protein, unsaturated fatty acids, carbohydrate, fibre and bioactive factors present in PhVa were comparable with those in other Phaseolus vulgaris varieties. However, the lectins of PhVa were predominantly of the leucoagglutinating type, and concentrated in the albumin protein fraction. Rats fed a diet (110 g total protein, 16·0 M/g) in which PhVa meal provided about half of the protein excreted high levels of N in faeces and urine, and grew more slowly, than rats fed a high-quality control diet (ad libitum or pair-fed). Small intestine, large intestine and pancreas weights were increased (by almost 100 %, P<0·05), whilst skeletal muscle, thymus and spleen weights were reduced. Blood insulin 16·20 v. 0·50 m/, P<0·05, thyroxine, glucose, protein (60·5 v. 48·3 /, P<0·05) and LDL-cholesterol were lowered, whilst glucagon (155·3 v. 185·4 n/, P<0·05), triiodothyronine and urea were elevated, as were urinary urea, creatinine and glucose. These changes in the local (gut) and systemic metabolism of rats were probably mediated primarily by lectins in PhVa, which were concentrated in the albumin protein fraction, whereas in many other Phaseolus vulgaris lines they are distributed across the globulin and albumin fractions.

Keywords

Phaseolus vulgaris LBlood compositionUrine compositionHormones
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 3 , March 2003 , pp. 311 - 318
Copyright
Copyright © The Nutrition Society 2003

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