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Enterocin HZ produced by a wild Enterococcus faecium strain isolated from a traditional, starter-free pickled cheese

Published online by Cambridge University Press:  24 February 2014

Zeliha Yildirim ,
Harun Bilgin ,
Hilal Isleroglu ,
Kader Tokatli ,
Didem Sahingil  and
Metin Yildirim
Zeliha Yildirim*
Affiliation:
Faculty of Engineering, Department of Food Engineering, Nigde University, Nigde, Turkey
Harun Bilgin
Affiliation:
Faculty of Engineering, Department of Food Engineering, Nigde University, Nigde, Turkey
Hilal Isleroglu
Affiliation:
Faculty of Engineering, Department of Food Engineering, Nigde University, Nigde, Turkey
Kader Tokatli
Affiliation:
Faculty of Engineering, Department of Food Engineering, Nigde University, Nigde, Turkey
Didem Sahingil
Affiliation:
Faculty of Engineering, Department of Food Engineering, Nigde University, Nigde, Turkey
Metin Yildirim
Affiliation:
Faculty of Engineering, Department of Food Engineering, Nigde University, Nigde, Turkey
*
*For correspondence; e-mail: zeliha.yildirim@nigde.edu.tr
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Abstract

Bacteriogenic Enterococcus faecium HZ was identified by using biochemical (Strep-API 20, API-50 CHL, fatty acid profile) and 16S rRNA analysis (99·99 %). Ent. faecium HZ was sensitive to clinically important antibiotics such as vancomycin, and did not have gelatinase and haemolysis activities. Enterocin HZ, a bacteriocin from Ent. faecium HZ, was sensitive to papain and tyripsin, but resistant to pepsin, lipase, catalase, α-amylase, organic solvents, detergents, ß-mercaptoethanol, and heat treatment (90 °C/30 min). It was biologically active at pH 2·0–9·0 and synthesised at the highest level in MRS or M17 broth at 32 or 37 °C with an inoculum amount of 0·1–0·5 % and an initial pH of 6·0–7·0. Enterocin HZ production reached maximum level at middle and late logarithmic phase and its molecular weight was ∼4·5 kDa. It was active against some Gram-positive foodborne bacteria. Ent. faecium HZ or its bacteriocin enterocin HZ is a good candidate to be studied as a food biopreservative since enterocin HZ showed strong bactericidal activity against Listeria monocytogenes in UHT milk and also Ent. faecium HZ grew very well in milk and produced enterocin HZ at maximum level.

Keywords

Bacteriocinenterocin HZEnterococcus faecium
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 2 , May 2014 , pp. 164 - 172
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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