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Bioactivity of water soluble extracts and some characteristics of white cheese during the ripening period as effected by packaging type and probiotic adjunct cultures

Published online by Cambridge University Press:  16 January 2015

Tuba Erkaya  and
Mustafa Şengul
Tuba Erkaya*
Affiliation:
Department of Food Engineering, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey
Mustafa Şengul
Affiliation:
Department of Food Engineering, Faculty of Agriculture, Atatürk University, 25240, Erzurum, Turkey
*
*For correspondence; e-mail: terkaya@atauni.edu.tr
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Abstract

In this study, the chemical composition, proteolysis and in vitro angiotensin-converting enzyme-(ACE)-inhibitory and antioxidant activities of white cheeses made using probiotic adjunct cultures (Bifidobacterium bifidum DSMZ 20456 and Lactobacillus acidophilus DSMZ 20079) were investigated. The cheeses were ripened in a vacuum package or brine for 120 d at 4 °C. The cheese samples maintained the probiotic characteristics of the viable cells as >106 cfu/g even after ripening for 120 d. The proteolysis degrees in terms of water-soluble nitrogen/total nitrogen (WSN/TN), trichloroacetic acid-soluble nitrogen/total nitrogen (TCA-SN/TN) and phosphotungstic acid-soluble nitrogen/total nitrogen (PTA-SN/TN) values in the cheeses increased throughout the ripening. The highest levels of proteolysis were found in cheese made using Lb. acidophilus DSMZ 20079 and ripened in a vacuum package. ACE-inhibitory activity of the water soluble extracts (WSEs) of the cheeses increased significantly (P < 0·05) throughout the ripening (IC50 values 82·78–140·99 μg/ml). Use of Lb. acidophilus DSMZ 20079 and packaging under vacuum significantly increased the percentage of ACE inhibiting activity. WSEs had DPPH scavenging activity (the IC50 values were 2·41–5·39 mg/ml and the inhibition values were 5·10–10·38%), increasing up to 60 d ripening. In the present study, it was observed that Lb. acidophilus DSMZ 20079 was more effective than Bifido. bifidum DSMZ 20456 in terms of the cheese characteristics investigated.

Keywords

White cheeseprobiotic adjunct culturevacuum packagingACE-inhibitory activity
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 1 , February 2015 , pp. 47 - 55
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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References

Akalın, AS & Karaman, AD 2011 Influence of packaging systems on the biochemical characteristics and volatile compounds of industrially produced Turkish White cheese. Journal of Food Biochemistry 35 663–680CrossRefGoogle Scholar
Alichanidis, E, Polychroniadou, A, Tzanetakis, N & Vafopoulou, A 1981 Teleme cheese from deep-frozen curd. Journal of Dairy Science 64 732–739Google Scholar
Apostolidis, E, Kwon, YI & Shetty, K 2007 Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Innovative Food Science and Emerging Technologies 8 46–54Google Scholar
Association of Official Analytical Chemists International 2000 Official Methods of Analysis, 17th edn.Gaitherburg, MD: AOAC InternationalGoogle Scholar
Bernardeau, M, Vernoux, JP, Henri-Dubernet, S & Gueguen, M 2008 Safety assessment of dairy microorganisms: the Lactobacillus genus. International Journal of Food Microbiology 126 278–285Google Scholar
Bütikofer, U, Meyer, J, Sieber, R, Walther, B & Wechsler, D 2008 Occurrence of the angiotensin-converting enzyme-inhibiting tripeptides Val-Pro-Pro and Ile-Pro-Pro in different cheese varieties of Swiss origin. Journal of Dairy Science 91 29–38Google Scholar
Fucà, N, McMahon, DJ, Caccamo, M, Tuminello, L, La Terra, S, Manenti, M & Licitra, G 2012 Effect of brine composition and brining temperature on cheese physical properties in Ragusano cheese. Journal of Dairy Science 95 460–470CrossRefGoogle ScholarPubMed
Ghoddusi, HB & Robinson, RK 1996 The test of time. Dairy Industries International 61 25–28Google Scholar
Gobbetti, M, Corsetti, A, Smacchi, E, Zocchetti, A & de Angelis, A 1998 Production of Crescenza cheese by incorporation of bifidobacteria. Journal of Dairy Science 81 37–47CrossRefGoogle Scholar
Gomes da Cruz, A, Buriti, FCA, Batista de Souza, CH, Faria, JAF & Saad, SMI 2009 Probiotic cheese: health benefits, technological and stability aspects. Trends in Food Science and Technology 20 344–354Google Scholar
Gómez-Ruiz, JÁ, Taborda, G, Amigo, L, Recio, I & Ramos, M 2006 Identification of ACE-inhibitory peptides in different Spanish cheeses by tandem mass spectrometry. European Food Research and Technology 223 595–601Google Scholar
Guinee, TP & Fox, PF 1993 Salt in cheese, physical, chemical and biological aspects. In Cheese: Chemistry, Physics and Microbiology, pp. 257–302 (Ed. Fox, PF). London, UK: Chapman & HallGoogle Scholar
Gupta, A, Mann, B, Kumar, R & Sangwan, RB 2009 Antioxidant activity of cheddar cheeses at different stages of ripening. International Journal of Dairy Technology 62 339–347Google Scholar
Gupta, A, Mann, B, Kumar, R & Sangwan, RB 2013 ACE-inhibitory activity of cheddar cheeses made with adjunct cultures at different stages of ripening. Advances in Dairy Research 1 2Google Scholar
Hayaloglu, AA, Guven, M & Fox, PF 2002 Microbiological, biochemical and technological properties of Turkish White cheese ‘Beyaz Peynir’. International Dairy Journal 12 635–648CrossRefGoogle Scholar
Hayaloglu, AA, Bansal, N & McSweeney, PLH 2012 Influence of brine immersion and vacuum packaging on the chemistry, biochemistry, and microstructure of Mihalic cheese made using sheep's milk during ripening. Dairy Science and Technology 92 671–689CrossRefGoogle Scholar
IDF 1993 International Dairy Federation, Standard Method 20B: Milk. Determination of Nitrogen Content. Brussels, Belgium: IDFGoogle Scholar
Jao, CL, Huang, SH & Hsu, KC 2012 Angiotensin I-converting enzyme inhibitory peptides: inhibition mode, bioavailability, and antihypertensive effects. BioMedicine 2 130–136Google Scholar
Jimsheena, VK & Gowda, LR 2009 Colorimetric, high-throughput assay for screening angiotensin I-converting enzyme inhibitors. Analytical Chemistry 81 9388–9394CrossRefGoogle ScholarPubMed
Kasımoğlu, A, Göncüoğlu, M & Akgün, S 2004 Probiotic white cheese with Lactobacillus acidophilus. International Dairy Journal 14 1067–1073Google Scholar
Kirkin, C, Gunes, G & Kilic-Akyilmaz, M 2013 Preservation of precut white cheese by modified atmosphere packaging. International Journal of Dairy Technology 66 576–586Google Scholar
Kuchroo, CN & Fox, PF 1982 Soluble nitrogen in Cheddar cheese: comparison of extraction procedures. Milchwissenschaft 37 331–335Google Scholar
Lima, KGDC, Kruger, MF, Behrens, J, Destro, MT, Landgraf, M & Gombossy de Melo Franco, BD 2009 Evaluation of culture media for enumeration of Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium animalis in the presence of subsp. bulgaricus and Streptococcus thermophilus. LWT-Food Science and Technology 42 491–495Google Scholar
Lowry, OH, Resebrough, NJ, Farr, AL & Randall, RJ 1951 Protein measurement with the folin phenol reagent. Journal of Biology and Chemistry 193 265–275CrossRefGoogle ScholarPubMed
McMahon, DJ, Motawee, MM & McManus, WR 2009 Influence of brine concentration and temperature on composition, microstructure, and yield of feta cheese. Journal of Dairy Science 92 4169–4179Google Scholar
McSweeney, PLH & Fox, PF 1997 Chemical methods for the characterization of proteolysis in cheese during ripening. Lait 77 41–76CrossRefGoogle Scholar
Meira, SMM, Daroit, DJ, Helfer, VE, Corrêa, APF, Segalin, S, Carro, S & Brandelli, A 2012 Bioactive peptides in water-soluble extracts of ovine cheeses from Southern Brazil and Uruguay. Food Research Internationl 48 322–329Google Scholar
Nielsen, MS, Martinussen, T, Flambard, B, Sørensen, KI & Otte, J 2009 Peptide profiles and angiotensin-I-converting enzyme inhibitory activity of fermented milk products: effect of bacterial strain, fermentation pH, and storage time. International Dairy Journal 19 155–165Google Scholar
Ong, L & Shah, NP 2008 Release and identification of angiotensin-converting enzyme inhibitory peptides as influenced by ripening temperatures and probiotic adjuncts in Cheddar cheeses. LWT – Food Science and Technology 41 1555–1566Google Scholar
Ong, L, Henriksson, A & Shah, NP 2007 Angiotensin converting enzyme-inhibitory activity in Cheddar cheeses made with the addition of probiotic Lactobacillus casei sp. Lait 87 149–165Google Scholar
Pappas, CP, Kondyli, E, Voutsinas, LP & Mallatou, H 1996 Effects of salting method and storage time on composition and quality of Feta cheese. International Journal of Dairy Technology 49 113–118Google Scholar
Pihlanto-Leppälä, A, Rokka, T & Korhonen, H 1998 Angiotensin I converting enzyme inhibitory peptides derived from bovine milk proteins. International Dairy Journal 8 325–331Google Scholar
Ryhänen, EL, Pihlanto-Leppälä, A & Pahkala, E 2001 A new type of ripened, low fat cheese with bioactive properties. International Dairy Journal 11 441–447Google Scholar
Sahingil, D, Hayaloglu, AA, Kirmaci, HA, Özer, B & Simsek, O 2014 Changes of proteolysis and angiotensin-I converting activity in white-brined cheese as affected by as affected by adjunct culture and ripening temperature. Journal of Dairy Research doi: 10.1017/S0022029914000326Google Scholar
Shapiro, SS & Wilk, MB 1965 An analysis of variance test for normality (complete samples). Biometrika 591–611Google Scholar
Smacchi, E & Gobbetti, M 1998 Peptides from several Italian cheeses inhibitory to proteolytic enzymes of lactic acid bacteria, Pseudomonas fluorescens ATCC 948 and to the angiotensin I converting enzyme. Enzyme and Microbial Technology 22 687–694Google Scholar
Stanton, C, Desmond, C, Coakley, M, Collins, JK, Fitzgerald, G & Ross, RP 2003 Challenges facing development of probiotic-containing functional foods. In Handbook of Fermented Functional Foods, pp. 50–79 (Ed. Farnworth, ER). Boca Raton: CRC PressGoogle Scholar
Tharmaraj, N & Shah, NP 2003 Selective enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus and propionibacteria. Journal of Dairy Science 86 2288–2296Google Scholar
Tonouchi, H, Suzuki, M, Uchida, M & Oda, M 2008 Antihypertensive effect of an angiotensin converting enzyme inhibitory peptide from enzyme modified cheese. Journal of Dairy Research 75 284–290Google Scholar
Vinderola, CG, Bailo, N & Reinheimer, JA 2000 Survival of probiotic microflora in Argentinian yoghurts during refrigerated storage. Food Research International 33 97–102CrossRefGoogle Scholar
Zhu, L, Chen, J, Tang, X & Xiong, YL 2008 Reducing, radical scavenging, and chelation properties of in vitro digests of alcalase-treated zein hydrolysate. Journal of Agricultural and Food Chemistry 56 2714–2721Google Scholar
Wang, HK, Dong, C, Chen, YF, Cui, LM & Zhang, HP 2010 A new probiotic cheddar cheese with high ACE-inhibitory activity and γ-Aminobutyric acid content produced with koumiss-derived Lactobacillus casei Zhang. Food Technology and Biotechnology 48 62–70Google Scholar