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Identification of a novel assemblage G subgenotype and a zoonotic assemblage B in rodent isolates of Giardia duodenalis in the Canary Islands, Spain

Published online by Cambridge University Press:  04 September 2013

ÁNGELA FERNÁNDEZ-ÁLVAREZ ,
AARÓN MARTÍN-ALONSO ,
NÉSTOR ABREU-ACOSTA ,
CARLOS FELIU ,
JEAN-PIERRE HUGOT ,
BASILIO VALLADARES  and
PILAR FORONDA
ÁNGELA FERNÁNDEZ-ÁLVAREZ
Affiliation:
University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain
AARÓN MARTÍN-ALONSO
Affiliation:
University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain
NÉSTOR ABREU-ACOSTA
Affiliation:
Analytical Biotech, Santa Cruz de Tenerife, Canary Islands, Spain
CARLOS FELIU
Affiliation:
Laboratory of Parasitology, Faculty of Pharmacy, University of Barcelona, Barcelona, Spain
JEAN-PIERRE HUGOT
Affiliation:
Muséum National d'Histoire naturelle, Paris, France
BASILIO VALLADARES
Affiliation:
University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain
PILAR FORONDA*
Affiliation:
University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, La Laguna, Tenerife, Canary Islands, Spain
*
*Corresponding author: University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna. Avda. Astrofísico Fco. Sánchez, s/n 38203 La Laguna, Canary Islands, Spain. E-mail: pforonda@ull.es
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Summary

The flagellated parasite Giardia duodenalis is known as one of the most common causes of protozoal diarrhoea in both humans and animals worldwide. The aim of the present work was to perform the first study of G. duodenalis in rodents in the Canary Islands (Spain) and analyse the level of genetic variation and the potential zoonotic role of the isolates. Stool samples were collected from 284 wild rodents and Giardia cysts were detected by light microscopy. The overall prevalence of giardiasis was 25·4% and ranged from 19·4% in El Hierro to 34% in Gran Canaria. Positive samples were further characterized by PCR and nucleotide sequencing of the triose phosphate isomerase (TPI), β-giardin (BG) and glutamate dehydrogenase (GDH) genes. Our study revealed assemblage G as the most frequent genotype and identified two rodent-infecting G. duodenalis haplotypes of this assemblage, HI and HII. Phylogenetic analysis supported the monophyly of haplotype HI, which we suggest to be considered as a novel G. duodenalis sub-assemblage GII, due to the high genetic distances among this sub-genotype and assemblage G. Furthermore, G. duodenalis assemblage B was detected in an inhabited area in La Palma, a fact that may pose a potential risk of G. duodenalis transmission from rodents to humans.

Keywords

Giardia duodenalisCanary Islandszoonosesrodentsassemblage Gsub-assemblage GII
Type
Research Article
Information
Parasitology , Volume 141 , Issue 2 , February 2014 , pp. 206 - 215
Copyright
Copyright © Cambridge University Press 2013 

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