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In vitro cytokines profile and ultrastructural changes of microglia and macrophages following interaction with Leishmania

Published online by Cambridge University Press:  09 April 2014

PATRICIA KARLA SANTOS RAMOS ,
MAYSA DE VASCONCELOS BRITO ,
FERNANDO TOBIAS SILVEIRA ,
CLÁUDIO GUEDES SALGADO ,
WANDERLEY DE SOUZA ,
CRISTOVAM WANDERLEY PICANÇO-DINIZ  and
JOSÉ ANTONIO JUNIOR PICANÇO-DINIZ
PATRICIA KARLA SANTOS RAMOS
Affiliation:
Instituto Evandro Chagas, Programa de Leishmanioses, Rod. BR-316, km 07, Bairro Levilândia, 67030-000, Ananindeua, Pará, Brazil
MAYSA DE VASCONCELOS BRITO
Affiliation:
Laboratório de Microscopia Eletrônica, Instituto Evandro Chagas, Av. Almirante Barroso 492, Bairro Marco, 66090-000, Belém, Pará, Brazil
FERNANDO TOBIAS SILVEIRA
Affiliation:
Instituto Evandro Chagas, Programa de Leishmanioses, Rod. BR-316, km 07, Bairro Levilândia, 67030-000, Ananindeua, Pará, Brazil
CLÁUDIO GUEDES SALGADO
Affiliation:
Laboratório de Dermato-Imunologia, Universidade do Estado do Pará (UEPA), Universidade Federal do Pará (UFPA), Unidade de Referência em Dermatologia Sanitária do Estado do Pará ‘Dr. Marcello Candia’ (MC), Brazil
WANDERLEY DE SOUZA
Affiliation:
Laboratório de Ultraestrutura Celular Hertha Meyer, Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Ilha do Fundão, Rio de Janeiro, RJ, Brazil
CRISTOVAM WANDERLEY PICANÇO-DINIZ
Affiliation:
Laboratório de Investigações em Neurodegeneração e Infecção, Universidade Federal do Pará, Hospital Universitário João de Barros Barreto, Brazil Hospital Universitário João de Barros Barreto, Rua Mundurucus, no. 4487, Lab de Investigações em Neurodegeneração e Infecção Guamá CEP 66073-000, Belém, PA, Brazil
JOSÉ ANTONIO JUNIOR PICANÇO-DINIZ*
Affiliation:
Laboratório de Microscopia Eletrônica, Instituto Evandro Chagas, Av. Almirante Barroso 492, Bairro Marco, 66090-000, Belém, Pará, Brazil
*
*Corresponding author: Laboratório de Microscopia Eletrônica, Instituto Evandro Chagas, Av. Almirante Barroso No 492, bairro Marco, Belém, Pará, CEP 66090-000, Brazil. E-mail: jose_apdj@yahoo.com.br
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Summary

In the present study, we assessed morphological changes and cytokine production after in vitro interaction with causative agents of American cutaneous leishmaniasis and compared the microglia and macrophage immune responses. Cultures of microglia and macrophages infected with stationary-phase promastigotes of Leishmania (Viannia) shawi, Leishmania (Viannia) braziliensis or Leishmania (Leishmania) amazonensis were evaluated 24, 48 and 72 h after interaction. Macrophages only presented the classical phagocytic process while microglia also displayed large cytoplasmic projections similar to the ruffles described in macropinocytosis. In the macrophage cultures, the percentage of infected cells increased over time, in a fashion that was dependent on the parasite species. In contrast, in microglial cells as the culture time progressed, there was a significant reduction in the percentage of infected cells independent of parasite species. Measurements of cytokines in macrophage cultures 48 h after interactions revealed distinct expression patterns for different parasites, whereas in microglial cultures they were similar for all Leishmania tested species. Taken together, our results suggest that microglia may have a higher phagocytic ability and cytotoxic potential than macrophages for all investigated species. The robust response of microglia against all parasite species may suggest microglia have an important role in the defence against cerebral leishmaniasis.

Keywords

Leishmaniamacrophagemicrogliainfectionultrastructurecytokines
Type
Research Article
Information
Parasitology , Volume 141 , Issue 8 , July 2014 , pp. 1052 - 1063
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Copyright
Copyright © Cambridge University Press 2014 

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