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Linking Microstructure and Nanochemistry in Human Dental Tissues

Published online by Cambridge University Press:  12 April 2012

Vesna Srot ,
Birgit Bussmann ,
Ute Salzberger ,
Christoph T. Koch  and
Peter A. van Aken
Vesna Srot*
Affiliation:
Stuttgart Center for Electron Microscopy, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Birgit Bussmann
Affiliation:
Stuttgart Center for Electron Microscopy, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Ute Salzberger
Affiliation:
Stuttgart Center for Electron Microscopy, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Christoph T. Koch
Affiliation:
Stuttgart Center for Electron Microscopy, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart, Germany
Peter A. van Aken
Affiliation:
Stuttgart Center for Electron Microscopy, Max Planck Institute for Intelligent Systems, Heisenbergstrasse 3, 70569 Stuttgart, Germany
*
Corresponding author. E-mail: srot@is.mpg.de
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Abstract

Mineralized dental tissues and dental pulp were characterized using advanced analytical transmission electron microscopy (TEM) methods. Quantitative X-ray energy dispersive spectroscopy was employed to determine the Ca/P and Mg/P concentration ratios. Significantly lower Ca/P concentration ratios were measured in peritubular dentine compared to intertubular dentine, which is accompanied by higher and variable Mg/P concentration ratios. There is strong evidence that magnesium is partially substituting calcium in the hydroxyapatite structure. Electron energy-loss near-edge structures (ELNES) of C-K and O-K from enamel and dentine are noticeably different. We observe a strong influence of beam damage on mineralized dental tissues and dental pulp, causing changes of the composition and consequently also differences in the ELNES. In this article, the importance of TEM sample preparation and specimen damage through electron irradiation is demonstrated.

Keywords

X-ray energy dispersive spectroscopyelectron energy-loss near-edge structuresCa/P and Mg/P concentration ratiosTEM sample preparationspecimen damagehydroxyapatitehuman dental tissues
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 3 , June 2012 , pp. 509 - 523
Copyright
Copyright © Microscopy Society of America 2012

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