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Genetic diversity of Colombian landraces of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites

Published online by Cambridge University Press:  04 March 2011

Lucy M. Díaz ,
Héctor F. Buendía ,
Myriam C. Duque  and
Matthew W. Blair
Lucy M. Díaz
Affiliation:
CIAT – International Center for Tropical Agriculture, AA6713, Cali, Colombia, South America
Héctor F. Buendía
Affiliation:
CIAT – International Center for Tropical Agriculture, AA6713, Cali, Colombia, South America
Myriam C. Duque
Affiliation:
CIAT – International Center for Tropical Agriculture, AA6713, Cali, Colombia, South America
Matthew W. Blair*
Affiliation:
CIAT – International Center for Tropical Agriculture, AA6713, Cali, Colombia, South America Cornell University, Department of Plant Breeding, Ithaca, New York, USA
*
*Corresponding author. E-mail: m.blair@cgiar.org
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Abstract

Colombia, situated at the northern end of the Andes mountains of South America and in proximity to Central America, is an important centre of diversity for common bean (Phaseolus vulgaris L.) that has a mix of cultivated germplasm from both major gene pools (Andean and Mesoamerican) for the species. Microsatellites are a useful marker system for analyzing genetic diversity of this crop and can be analyzed with manual (silver-stain) or automated (ABI) detection systems and using unlabelled or fluorescently labelled markers, respectively. The objectives of this research were to evaluate the genetic diversity of 92 Colombian landraces and gene pool controls with 36 fluorescent and 30 non-fluorescent microsatellite markers and to determine the extent of introgression between the Andean and Mesoamerican gene pools for this germplasm. A comparison of fluorescent versus non-fluorescent marker systems was performed with 14 loci, which were evaluated with both methods; the fluorescent markers were found to be more precise than the non-fluorescent markers in determining population structure. A combined analysis of 52 microsatellites using the 36 fluorescent markers and 16 non-overlapping, silver-stained markers produced an accurate population structure for the Andean gene pool that separated race Nueva Granada and race Peru genotypes and clearly identified introgression between these races and the gene pools. The results of this research are important for the application of microsatellite markers to diversity analysis in common bean and for the conservation of landraces in Colombia and neighbouring countries of Latin America, where similar germplasm exists and where gene pool or race mixtures also occur.

Keywords

genetic diversity indicespopulation structureprincipal coordinate analysissimple sequence repeats
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 1 , April 2011 , pp. 86 - 96
Copyright
Copyright © NIAB 2010

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